Description efficient gas delivery while minimizing excess gas exposure to lab personnel. Turn on the flow of gas to the ivis manifold with toggle 7. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics. Computational vision and medical image processing computational methods in applied sciences volume 19 series editor e. Caliper life sciences xenogen xgi8 by caliper life sciences.
Xenogen gas anesthesia system model xgi 8 with warranty. Prior to imaging, the mice were anesthetized using the xgi8 gas anesthesia system caliper life sciences, inc. Perkinelmers xgi8 gas anesthesia system is designed to work with ivis. The system is equipped with filter sets to image reporters that emit from green to nearinfrared, an xgi 8 gas anesthesia system, and can image up to 5 mice at a time. Heatactivated spore suspensions of various concentrations see below in 25.
See page 31 for more details on the use of the transfer bed and mouse imaging shuttle. Xgi8 gas anesthesia system for ivis preclinical in. System imaging chamber is maintained with a 5port or 2port anesthesia manifold. Computational vision and medical image processing pdf free. Place the animals in the induction box 6, and wait till the desired level of anesthesia is reached. The xenogen xgi8 gas anesthesia system is designed to work with the ivis imaging system, a technology from xenogen that allows researchers to use r ealtime in vivo imaging to monitor and record cellular and genetic activity within a living organism. The system is equipped with filter sets to image reporters that emit from green to nearinfrared, an xgi8 gas anesthesia system, and can image up to 5 mice at a time.
Do not touch or expose the four diffusing reflectors and the exposed emission filter to contaminants described in system components, page 35 and shown in figure 9. Xgi8 gas anesthesia isoflurane vaporizer with independent flow control for proper gas delivery to induction chamber and the ivis imaging system imaging chamber isofluraneabsorbing disposable charcoal filters to absorb excess gas, as well as limit gas escaping into the surrounding laboratory environment. Secreted luciferase for in vivo evaluation of systemic protein delivery in mice. Detection of bacillus anthracis spore germination in vivo by. Caliper recommends using the xgi 8 gas anesthesia system when imaging small animals. Vetequip assumes no liability for any malfunction, failure, damage or loss to either equipment or life. The cornerstone of the innate immune response is the diverse group of cells, including macrophages, neutrophils, and lymphocytes, that contribute to host defense through the recognition, isolation, and eradication of pathogens.
Use only oxygen regulated to 5055 psi as the carrier gas. Perkinelmer caliper ivis spectrum hardware manual pdf download. Four superoxide dismutases contribute to bacillus anthracis. The ras4 successfully facilitates efficient gas delivery via four delivery circuits. Chromosomal mutagenesis, edited by greg davis and kevin j. The system is a small portable unit that is designed for maximum efficiency, ease of use and low anesthetic gas consumption. All other trademarks are the property of their respective owners. An integrated gas isoflurane anesthesia xgi8 anesthesia system, allows for repeated daily use in rodents. Caliper life sciences xenogen xgi8 community, manuals. Hematopoietic stem cell protocols pdf free download. Mice were under continuous anesthesia on a heated stage using the xgi8 gas anesthesia system. The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense. Secreted luciferase for in vivo evaluation of systemic. Hoggan scientific microfet2 handheld manual muscle tester kit w warranty.
Perkinelmers xgi8 gas anesthesia system is designed to work with ivis imaging systems and allows researchers to use realtime. This instrument may also be used for fluorescent imaging of gels or cell culture plates. Avian influenza virus, edited by erica spackman, 2008 435. Welcome 4 the system takes very lowlight leve l images, stores them, and displays. The xgi8 anesthesia system is used in conjunction with the ivis spectrum to anesthetize mice undergoing bioluminescent or fluorescent whole body imaging.
Welcome camera xgi8 gas anesthesia system high resolution monitor imaging chamber acquisition computer xws260 workstation compartment that contains the thermocube chiller unit and camera power supply figure 1. The xgi8 gas anesthesia system delivers and actively scavenges gas during the imaging process. The standard mounting system requires bolting of the vaporizer directly to a rigid back bar of an anesthesia gas machine. The xgi 8 anesthesia module is the main device that controls the delivery of. Perkinelmer s xgi8 gas anesthesia system is designed to work with ivis imaging systems and allows researchers to use realtime.
Using the xgi8 gas anesthesia system university of houston. Caliper life sciences mobilityshift optimization perkinelmer caliper life sciences mobilityshift optimization. Xgi8 gas anesthesia system contact information please call 1. It facilitates efficient gas delivery via four delivery circuits while minimizing excess gas exposure to lab personnel with active scavenging capabilities.
The xenogen xgi8 gas anesthesia system is designed to work with the ivis imaging system, a technology from xenogen that allows researchers to use realtime in vivo imaging to monitor and record cellular and genetic. The ivis200 bioluminescence imaging machine was equipped with an xgi8 gas anesthesia system, and isoflurane butler schein, 029404 with a flow rate of 2. These isofluraneabsorbing charcoal filters are made for use with xgi8 anesthesia system. For intranasal spore challenge, mice were sedated with isoflurane with an xgi8 gas anesthesia system xenogen corporation. Introduction to the ibox anesthesia system the ibox anesthesia system is designed for the anesthesia of small animals. A lighttight imaging chamber for quantitative bioluminescent and fluorescent imaging in vivo and in vitro. We specialize in buying and selling high quality, new and used laboratory and pharmaceutical equipment. The gas anesthesia module successfully integrates e. Prior to imaging, the mice were anesthetized using the xgi 8 gas anesthesia system caliper life sciences, inc. The xgi8 gas anesthesia system is intended for use with ivis imaging systems to anesthetize up to 10 laboratory mice prior to and during imaging. The xgi 8 gas anesthesia system delivers and then minimizes exposure to excess gas during the imaging process. Detection of bacillus anthracis spore germination in vivo. If gas anesthesia is to be used, the xgi 8 gas anesthesia system and the mouse imaging shuttle are recommended.
Animals were injected intraperitoneally 5 minutes prior to imaging with luciferin 150mgkg of body weight, 1mg dluciferin in 1ml of 2x pbs, xenogen, xr1001. Bioluminescence imaging was obtained by acquiring photon counts over 5 min immediately after intravenous tailvein injection of 150 l coelenterazine 4 gg body weight using a 26 mm square backthinned ccd camera. Realtime in vivo imaging of transgenic bioluminescent. The xgi8 system continuously removes the waste anesthesia gas mixture from the area around the anesthetized mice to reduce, though not eliminate, user. The xgi8 gas anesthesia system is designed to work with ivis imaging systems and allows researchers to use realtime in vivo imaging to monitor and record cellular and genetic activity within a living organism the gas anesthesia module successfully integrates efficient gas. Hypoxisense is a fluorescent dye which detects surface expression of carboxic anhydrase 9.
The xgi 8 system continuously removes the waste anesthesia gas mixture from the area around the anesthetized mice to reduce, though not eliminate, user exposure to the isoflurane anesthetic agent. Inspectturns the fluorescent lamp on manually, allowing the user to inspect the action inside the ivis. This protocol describes a methodology for imaging the sequestration of infected erythrocytes of the rodent malaria parasite plasmodium berghei in. Pdf secreted luciferase for in vivo evaluation of systemic. Ensure adequate ventilation before using gas anesthesia equipment. The xgi8 anesthesia module is the main device that controls the delivery of. Efficient gas delivery while minimizing excess gas exposure to lab personnel. Initialize the system, wait for the camera to cool down till the temperature button turns green. A heated stage, to help maintain body temperature during imaging. Before turning on the xgi8 anesthesia machine it is important to checkdo the following.
Mice or rats are anesthesized in the induction chamber prior to imaging. If you have questions regarding this manual or the ivis imaging system, please. Ivis spectrum 7 ivis spectrum complete imaging solutions accessories reagents anesthesia system cat no. The xenogen xgi8 gas anesthesia system is designed to work with the ivis imaging system, a technology from xenogen that allows researchers to use r. Nanoparticle mediated microvascular embolization nme of tumor tissue may occur after systemic administration of pem, leading to widespread shutdown of vascular flow, hemorrhage, and necrosis. Living lmage software version 2 cornell university.
Imaging was performed using an ivis spectrum optical imaging system fitted with an xgi8 gas anesthesia system caliper life sciences, hopkinton, ma. Before turning on the xgi8 anesthesia machine it is important to checkdo the following weigh the fair canisters. Gas anesthesia ports and 3 or 5 position manifold within imaging chamber allow anesthesia to be maintained during imaging sessions syringe injection system, integrated with living image, allows the user to acquire real time functional responses to compounds ivis lumina xr a b a b exchangeable filter wheel shielded cabinet xray module. The anesthesia system allows researchers to use realtime. Computational vision and medical image processing pdf.
Pleasanton, ca usa 8004666463 5 for veterinary use only. Ivis spectrum imaging core research technology support facility. The xgi8 system continuously removes the waste anesthesia gas mixture from the area around the anesthetized mice to reduce, though not eliminate, user exposure to the isoflurane anesthetic agent. The ivis200 bioluminescence imaging machine was equipped with an xgi 8 gas anesthesia system, and isoflurane butler schein, 029404 with a flow rate of 2. Ivis spectrum imaging core research technology support. Select a user id from the dropdown list or enter a new user id up to three letters, and click ok 7. Perkinelmer caliper ivis spectrum hardware manual pdf. Always adhere to proper diameter index safety system d. The xenogen xgi8 gas anesthesia system is designed to work with the ivis imaging system, a technology from xenogen that allows researchers to use realtime in vivo imaging to monitor and record cellular and genetic activity within a living organism. It facilitates efficient gas delivery via four delivery circuits while minimizing excess gas exposure to lab personnel with active scavenging. Unless otherwise specified, all vaporizers are supplied standard with 23mm inlet and outlet tapers. Anesthesia gas machine caliper life sciences xenogen xgi8.
Mar 11, 2012 secreted luciferase for in vivo evaluation of systemic protein delivery in mice. The ras4 rodent anesthesia system is compatible with our suite of in vivo preclinical imaging systems including ivis and fmt optical imaging systems as well as the quantum microct imaging system. Click on our partners to see what last minute vacation destinations they airline jobs in massachusetts for the how to study for mrcp part 1 three years, sunwing has been listed by profit magazine as one of. The xgi 8 gas anesthesia system is intended for use with ivis imaging systems to anesthetize up to 10 laboratory mice prior to and during imaging. An integrated gas isoflurane anesthesia xgi 8 anesthesia system, allows for repeated daily use in rodents. Distributor agreement between xenogen corporation 860. The gas anesthesia module successfully integrates efficient gas delivery while minimizing. The ivis imaging system 100 series and the xgi8 gas anesthesia system. The xgi 8 anesthesia system is used in conjunction with the ivis spectrum to anesthetize mice undergoing bioluminescent or fluorescent whole body imaging. Xgi8 gas anesthesia system for ivis preclinical in vivo. Compositions and methods for inducing nanoparticle. Described herein are compositions and methods for treating, inhibiting, preventing, reducing the severity of andor reducing the progression of schwannoma, as well as any disease or physiological diseasestate in which schwannoma plays a role. Caliper life sciences xenogen xgi8 community, manuals and. Hematopoietic stem cell protocols m e t h o d s i n m o l e c u l a r b i o l o g ytm john m.
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